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There is no direct connection between Kiev and Prague.
Kiev 11:45 – Vienna 11.21 (the next day) → Vienna 13.10 – Prague 17.41
Price from 142 €
Kiev 17:00 – Przemysl 05:00 (the next day) → Przemysl 07:10 – Prague 17:52
Price from 96 €
Tickets can be ordered here (order the 2 trains separately). Reservations open 2–3 weeks before departure.
Hotels in Kiev (Booking.com)
Hotels in Prague (Booking.com)