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There is no direct train connection.
We recommand taking a bus bus from Uzhhorod to Košice and then change to a night train from Košice to Prague.
Uzhhorod 16.10 – Košice 18.15 → Košice 21.22 – Prague 05.54 (next day)
Hotels in Uzhhorod (Booking.com)
Hotels in Prague (Booking.com)